Rob Sturm's List: Mass Spectrometry tidbits

• 200-1000 pmol, for a 50 kDa protein this is 10 to 50 µg.
• ith capillary chromatography a chromatographic or LC/MS query is easily accomplished with just 5 pmol
• Absorptive losses to large surface areas is one of the major hurdles in micro characterization
• ince mass spectrometers are concentration-dependant detectors the increased peak concentrations gave mass spectrometers more than just a suitable flow rate for LC/MS analysi

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